A novel strategy for the isolation of luxI homologues: evidence for the widespread distribution of a LuxR:LuxI superfamily in enteric bacteria

Mol Microbiol. 1993 Nov;10(3):511-20. doi: 10.1111/j.1365-2958.1993.tb00923.x.

Abstract

The pheromone N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) regulates expression of bioluminescence in the marine bacterium Vibrio fischeri, the production of carbapenem antibiotic in Erwinia carotovora and exoenzymes in both E. carotovora and Pseudomonas aeruginosa. A characteristic feature of this regulatory mechanism in V. fischeri is that it is cell density-dependent, reflecting the need to accumulate sufficient pheromone to trigger the induction of gene expression. Using a lux plasmid-based bioluminescent sensor for OHHL, pheromone production by E. carotovora, Enterobacter agglomerans, Hafnia alvei, Rahnella aquatilis and Serratia marcescens has been demonstrated and shown also to be cell density-dependent. Production of OHHL implies the presence in these bacteria of a gene equivalent to luxI. Chromosomal banks from all five enteric bacteria have yielded clones capable of eliciting OHHL production when expressed in Escherichia coli. The luxI homologue from both E. carotovora (carI) and E. agglomerans (eagI) were characterized at the DNA sequence level and the deduced protein sequences have only 25% identity with the V. fischeri LuxI. Despite this, carI, eagI and luxI are shown to be biologically equivalent. An insertion mutant of eagI demonstrates that this gene is essential for OHHL production in E. agglomerans.

Publication types

  • Comparative Study

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / physiology
  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Cloning, Molecular
  • Enterobacteriaceae / genetics*
  • Enterobacteriaceae / metabolism
  • Gene Expression Regulation, Bacterial*
  • Genes, Bacterial*
  • Genes, Regulator*
  • Genetic Complementation Test
  • Homoserine / analogs & derivatives*
  • Homoserine / physiology
  • Luciferases / biosynthesis
  • Luminescent Measurements
  • Molecular Sequence Data
  • Multigene Family
  • Recombinant Fusion Proteins / metabolism
  • Regulon
  • Repressor Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Trans-Activators*
  • Transcription Factors / genetics*
  • Transformation, Bacterial
  • Vibrio / genetics*
  • Vibrio / metabolism

Substances

  • Bacterial Proteins
  • LuxI protein, Bacteria
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Trans-Activators
  • Transcription Factors
  • LuxR autoinducer binding proteins
  • N-(beta-oxooctan-1-oyl)homoserine lactone
  • Homoserine
  • N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone
  • Luciferases
  • 4-Butyrolactone

Associated data

  • GENBANK/X74299
  • GENBANK/X74300