Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase

Photochem Photobiol. 1994 Sep;60(3):225-30. doi: 10.1111/j.1751-1097.1994.tb05095.x.


The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Bacterial Agents / radiation effects*
  • Blotting, Southern
  • Cell Line
  • Cell Survival / drug effects
  • Coumarins
  • DNA / drug effects*
  • DNA, Ribosomal / drug effects
  • DNA-Binding Proteins / drug effects*
  • Fanconi Anemia
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Genes / drug effects*
  • Glycosides
  • Humans
  • Intercalating Agents / pharmacology*
  • Methoxsalen / pharmacology
  • Photochemistry
  • Photosensitizing Agents / pharmacology*
  • RNA, Ribosomal / genetics*
  • Tetrahydrofolate Dehydrogenase / genetics*
  • Xeroderma Pigmentosum


  • Aminoglycosides
  • Anti-Bacterial Agents
  • Coumarins
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Glycosides
  • Intercalating Agents
  • Photosensitizing Agents
  • RNA, Ribosomal
  • gilvocarcin V
  • DNA
  • Tetrahydrofolate Dehydrogenase
  • Methoxsalen