Isolation and Quantitation of Long-Chain Acyl-Coenzyme A Esters in Brain Tissue by Solid-Phase Extraction

Anal Biochem. 1994 Aug 1;220(2):321-3. doi: 10.1006/abio.1994.1344.

Abstract

Long-chain acyl-CoA's are important intermediates in fatty acid oxidation and phospholipid metabolism. For quantitative analysis of brain acyl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrolase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes solid-phase extraction by an oligonucleotide purification cartridge and HPLC measurements using a synthetic internal standard. Estimates of concentration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6.0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl- and arachidonoyl-CoA (2.0 nmol/g) for a total concentration of 23 nmol/g brain.

MeSH terms

  • Acyl Coenzyme A / analysis*
  • Acyl Coenzyme A / isolation & purification*
  • Animals
  • Brain Chemistry*
  • Chromatography, High Pressure Liquid / methods
  • Palmitoyl Coenzyme A / analysis
  • Palmitoyl Coenzyme A / isolation & purification
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Acyl Coenzyme A
  • arachidonyl-coenzyme A
  • oleoyl-coenzyme A
  • Palmitoyl Coenzyme A
  • stearoyl-coenzyme A
  • linoleoyl-coenzyme A