Laboratory evaluation of the DCA 2000 clinic HbA1c immunoassay analyser

Ann Clin Biochem. 1994 Jul;31 ( Pt 4):367-70. doi: 10.1177/000456329403100411.

Abstract

The DCA 2000 clinical analyser for the measurement of haemoglobin A1c was evaluated for analytical quality. The analyser, which utilises inhibition of latex agglutination immunoassay, demonstrated good within-batch (1.9-3.1% CV) and between-batch (2.2% CV) imprecision, and was not affected by haemoglobin concentration. The analyser was linear throughout the analytical range, and was found to correlate well with agar electroendosmosis (r = 0.93), affinity chromatography (r = 0.97), HPLC (r = 0.90) and EIA (r = 0.98). The analyser was found to give reliable analytical results, and with its ease of use, will provide the diabetologist with HbA1c results in the clinic; although an analysis time of 9 min will limit sample throughput.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Blood Proteins / analysis
  • Blood Proteins / metabolism
  • Blood Specimen Collection
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Diabetes Mellitus / blood
  • Diabetes Mellitus / diagnosis*
  • Electrophoresis, Agar Gel
  • Evaluation Studies as Topic
  • Glycated Hemoglobin A / analysis*
  • Glycated Hemoglobin A / metabolism
  • Hemoglobins / analysis
  • Humans
  • Immunoassay / methods
  • Immunoassay / standards
  • Immunoenzyme Techniques
  • Latex Fixation Tests
  • Osmosis
  • Quality Control
  • Reproducibility of Results

Substances

  • Antibodies, Monoclonal
  • Blood Proteins
  • Glycated Hemoglobin A
  • Hemoglobins