Adenoviruses Isolated in the Stockholm Area During 1987-1992: Restriction Endonuclease Analysis and Molecular Epidemiology

Arch Virol. 1994;137(1-2):101-15. doi: 10.1007/BF01311176.

Abstract

Adenoviruses recovered from the northern Stockholm area during 1987-1992 have been subjected to restriction endonuclease analysis. Adenoviruses of all subgenera (A-F) were represented and a considerable degree of serotype variation was seen, e.g. the rarely encountered subgenus A viruses were frequently isolated in the present study. Of 16 subgenus A isolates, Ad31 predominated with 12 strains which were equally distributed into the DNA-variants D2 and D7. Analysis of 38 Ad3 isolates revealed four DNA-variants: D1, D3, D10, and "Sto1". The ten Ad7 isolates belonged all to the DNA-variant D5 of Ad7. Of 27 Ad1 strains, 11 belonged to D10, followed by the DNA-variants D4 and D7 with four strains each. Among Ad2 isolates, D2 or D2-like strains prevailed (23/28). Of six Ad5 strains, three belonged to the DNA-variant D2. The most notable subgenus D event was a nosocomial outbreak of keratokonjunctivitis due ot Ad19a. In addition, a collection of heterogenous subgenus D strains was detected, most of which untypable by RE-analysis. Among the six Ad4 isolates of subgenus E, both genomic clusters (p and a, respectively) of Ad4 were recognized. Concerning the clinical important subgenus F adenoviruses, only two strains of Ad40 were detected as compared to 12 strains of Ad41, all of which ascribed to the DNA-variant D12 of Ad41.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenovirus Infections, Human / epidemiology
  • Adenovirus Infections, Human / virology*
  • Adenoviruses, Human / classification*
  • Adenoviruses, Human / genetics
  • Adenoviruses, Human / isolation & purification*
  • Cross Infection / virology
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Genetic Variation
  • HeLa Cells
  • Humans
  • Keratoconjunctivitis / virology
  • Restriction Mapping
  • Serotyping
  • Sweden
  • Urban Population*

Substances

  • DNA, Viral