Mechanistic features associated with induction of metalloproteinases in human gingival fibroblasts by interleukin-1

Arch Oral Biol. 1994 Aug;39(8):657-64. doi: 10.1016/0003-9969(94)90091-4.


Human gingival fibroblasts were treated with recombinant interleukin-1 (IL-1) to determine the effect of this stimulus on the relative expression of collagenase (MMP-1), stromelysin (MMP-3) and plasminogen activator (PA) mRNA. The steady-state mRNA levels for these genes were determined on Northern blots. IL-1 induced steady-state levels of these mRNAs to different extents. Nuclear run-on transcription studies showed that IL-1 induction of neutral metalloproteinase may be transcriptionally regulated. Actinomycin D and protein kinase inhibitors decreased the mRNA production for all three metalloproteinases, whereas cycloheximide decreased the production of collagenase and stromelysin mRNA. Protein kinase inhibitors (H7/H8) decreased production of the three mRNAs to different extents. This study demonstrates a potentially important role for IL-1 in the regulation of metalloproteinase expression in human gingival fibroblasts. The ability of IL-1 to induce the expression of stromelysin, collagenase and PA may define a pivotal role for this cytokine in the pathogenesis of periodontitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cells, Cultured
  • Collagenases / biosynthesis
  • Collagenases / genetics
  • Cycloheximide / pharmacology
  • Enzyme Induction / drug effects
  • Enzyme Induction / genetics
  • Fibroblasts / enzymology*
  • Gingiva / cytology
  • Gingiva / enzymology*
  • Humans
  • Interleukin-1 / physiology*
  • Matrix Metalloproteinase 1
  • Matrix Metalloproteinase 3
  • Metalloendopeptidases / biosynthesis*
  • Metalloendopeptidases / genetics
  • Plasminogen Activators / biosynthesis
  • Plasminogen Activators / genetics
  • Protein Kinase Inhibitors
  • RNA, Messenger / analysis
  • Recombinant Proteins / pharmacology
  • Transcription, Genetic


  • Interleukin-1
  • Protein Kinase Inhibitors
  • RNA, Messenger
  • Recombinant Proteins
  • Cycloheximide
  • Plasminogen Activators
  • Collagenases
  • Metalloendopeptidases
  • Matrix Metalloproteinase 3
  • Matrix Metalloproteinase 1