Detection of an NAD(+)-dependent malic enzyme locus in the Atlantic salmon Salmo salar and other salmonid fish

Biochem Genet. 1994 Apr;32(3-4):105-17. doi: 10.1007/BF00554419.

Abstract

Electrophoretic studies of malate oxidoreductases routinely assess variation in two enzymes, malate dehydrogenase (EC 1.1.1.37) and malic enzyme (NADP+) (EC 1.1.1.40). By modification of the standard isozyme staining conditions for these enzymes, we have resolved a new NAD(+)-preferring, MgCl2-requiring malic enzyme which is indicated to be EC 1.1.1.39. The enzyme was detected in 10 salmonid fish species of the genera Salmo, Salvelinus, and Onchorynchus. Phenotypic variation indicates that the novel enzyme is tetrameric and coded by a single locus. Inheritance in S. salar follows a single-locus model and the phenotypes are unlinked to polymorphisms for sMDH-3,4* and mMEP-2*, two malate oxidoreductase loci previously shown to be variable in this species.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Frequency
  • Genes*
  • Genetic Markers
  • Genetic Variation
  • Isoenzymes / genetics*
  • Malate Dehydrogenase / genetics*
  • NAD / physiology*
  • Phenotype
  • Salmon / genetics*
  • Salmon / metabolism
  • Salmonidae / genetics*
  • Salmonidae / metabolism
  • Species Specificity

Substances

  • Genetic Markers
  • Isoenzymes
  • NAD
  • Malate Dehydrogenase
  • malate dehydrogenase (decarboxylating)