The tetratricopeptide repeat-domain of the PAS10 protein of Saccharomyces cerevisiae is essential for binding the peroxisomal targeting signal-SKL

Biochem Biophys Res Commun. 1994 Nov 15;204(3):1016-22. doi: 10.1006/bbrc.1994.2564.

Abstract

The PAS10 gene was found in a two-hybrid screen for the isolation of genes encoding proteins which interact with the C-terminal peroxisomal targeting signal -SKL. The PAS10 protein is known to be involved in import of proteins into peroxisomes and to contain a tetratricopeptide repeat (TPR) domain. All TPR-containing proteins involved in diverse processes like mitosis or RNA-synthesis share the ability to interact with other proteins. Here we show that the PAS10 protein interacts in vivo with the C-terminal peroxisomal targeting signal. The part essential for this interaction contains the complete tetratricopeptide repeat domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / metabolism*
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / metabolism*
  • Genes, Fungal
  • Membrane Transport Proteins*
  • Microbodies / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Peroxisome-Targeting Signal 1 Receptor
  • Plasmids
  • Protein Sorting Signals / chemistry
  • Protein Sorting Signals / metabolism
  • Repetitive Sequences, Nucleic Acid
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins

Substances

  • Carrier Proteins
  • Fungal Proteins
  • Membrane Transport Proteins
  • PEX5 protein, S cerevisiae
  • Peroxisome-Targeting Signal 1 Receptor
  • Protein Sorting Signals
  • Saccharomyces cerevisiae Proteins