Methods for the detection of apoptosis

Int Arch Allergy Immunol. 1994 Dec;105(4):327-32. doi: 10.1159/000236777.


Apoptosis is central to many basic and clinically oriented investigations, and this article is a brief overview of the most frequently utilized methods for detection of apoptotic cells, including the study of morphology, analysis of DNA degradation, DNA end labeling techniques, flow cytometric analysis, and nuclease assays. Features and advantages of the different methods are discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Apoptosis*
  • B-Lymphocytes / pathology
  • B-Lymphocytes / physiology
  • DNA / metabolism
  • Flow Cytometry
  • Humans
  • Necrosis
  • T-Lymphocytes / pathology
  • T-Lymphocytes / physiology


  • DNA