Penetrating keratoplasty using 37 C organ cultured cornea

Trans Sect Ophthalmol Am Acad Ophthalmol Otolaryngol. 1976 Sep-Oct;81(5):778-93.


Sixty-three penetrating keratoplasties were performed in 63 patients using donor corneas stored by 37 C organ culture incubation for an average of 13 days (range 2 to 35 days). No grafts failed before four weeks, except one which was stored at 4 C for 48 hours prior to transplantation, indicating that endothelial viability is maintained when proper storage conditions are maintained. Sixteen grafts have failed as a result of factors other than the storage method. This clinical study confirms our previous laboratory conclusions that 37 C OC incubation up to 35 days improves the quality of donor endothelium compared with other methods of storage, and it represents a significant advance in corneal preservation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Body Temperature
  • Child
  • Child, Preschool
  • Cornea / anatomy & histology
  • Corneal Transplantation*
  • Endothelium / physiology
  • Evaluation Studies as Topic
  • Follow-Up Studies
  • Graft Rejection
  • Humans
  • Middle Aged
  • Organ Culture Techniques / methods*
  • Organ Preservation / methods
  • Transplantation, Homologous