Pax-QNR/Pax-6, a paired box- and homeobox-containing gene expressed in neurons, is also expressed in pancreatic endocrine cells

Mol Endocrinol. 1994 Jul;8(7):929-38. doi: 10.1210/mend.8.7.7984154.


After differential screening of a cDNA library constructed from quail neuroretina cells infected with the v-myc containing avian retrovirus MC29, we have isolated a cDNA clone Pax-QNR, homologous to the murine Pax6 which is mutated in the autosomal dominant mutation small eye (Sey) of the mouse and aniridia in man. Here we report the characterization of Pax-QNR/Pax-6 expression in the chicken, quail, and mouse pancreas. In situ hybridization performed with E3 chick embryos demonstrated that, in addition to the documented expression of Pax-QNR/Pax-6 in the neural tube, this gene is also expressed in the pancreatic bud. This expression is later restricted to discrete parts of the organ. From bacterially expressed Pax-QNR peptides we obtained rabbit antisera (paired domain, serum 11; domain between paired and homeo, serum 12; homeodomain, serum 13; and carboxyl-terminal part, serum 14) capable of specifically recognizing Pax-QNR/Pax-6 proteins (48, 46 kilodaltons) in cell lines derived from alpha- and beta-pancreatic cells, but not from exocrine derived cell lines. We conclude that Pax-QNR/Pax-6 represents another gene expressed both in the endocrine pancreas and neuro-ectodermic tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Base Sequence
  • Cell Line, Transformed
  • Chick Embryo
  • Chickens / genetics
  • Coturnix / genetics
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / immunology
  • Eye Proteins / biosynthesis
  • Eye Proteins / genetics*
  • Gene Expression
  • Genes, Dominant
  • Genes, Homeobox*
  • Homeodomain Proteins*
  • Immune Sera
  • Islets of Langerhans / embryology
  • Islets of Langerhans / growth & development
  • Islets of Langerhans / metabolism*
  • Mice / genetics
  • Mice / growth & development
  • Mice, Transgenic
  • Molecular Sequence Data
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Neurons / metabolism*
  • Organ Specificity
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Pancreatic Neoplasms / pathology
  • Peptide Fragments / immunology
  • Polymerase Chain Reaction
  • Repressor Proteins
  • Retina / cytology
  • Species Specificity
  • Tumor Cells, Cultured


  • DNA, Complementary
  • DNA-Binding Proteins
  • Eye Proteins
  • Homeodomain Proteins
  • Immune Sera
  • Neoplasm Proteins
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Pax6 protein, mouse
  • Peptide Fragments
  • Repressor Proteins