Crystallization and preliminary X-ray diffraction studies of mandelonitrile lyase from almonds

Proteins. 1994 Aug;19(4):343-7. doi: 10.1002/prot.340190410.


Single crystals of three different isoenzymes of (R)-(+) mandelonitrile lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have been obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 69.9, b = 95.1, c = 95.6 A, and beta = 118.5 degrees. A complete set of diffraction data has been collected to 2.6 A resolution on native crystals of isoenzyme III.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde-Lyases / chemistry*
  • Aldehyde-Lyases / isolation & purification
  • Crystallography, X-Ray
  • Isoenzymes / chemistry
  • Isoenzymes / isolation & purification
  • Nuts / enzymology*


  • Isoenzymes
  • Aldehyde-Lyases
  • mandelonitrile lyase