Reconstitution of an operational MHC class II compartment in nonantigen-presenting cells

Science. 1994 Dec 2;266(5190):1569-73. doi: 10.1126/science.7985028.


Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain (li), and H-2M, the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal compartment, not at the cell surface, and the localization was independent of li. The data presented show that H-2M, class II, and li are the minimally required components for efficient formation of stable class II-peptide complexes, and thus for a functional class II compartment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigen Presentation*
  • Antigen-Presenting Cells / immunology
  • Antigens, Differentiation, B-Lymphocyte*
  • B-Lymphocytes / immunology
  • Cell Line
  • Cell Membrane / immunology
  • Endosomes / immunology*
  • Fluorescent Antibody Technique
  • H-2 Antigens / analysis
  • H-2 Antigens / genetics
  • H-2 Antigens / metabolism*
  • HLA-DR3 Antigen / metabolism*
  • HeLa Cells
  • Histocompatibility Antigens Class II / metabolism*
  • Humans
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Transfection


  • Antigens, Differentiation, B-Lymphocyte
  • H-2 Antigens
  • HLA-DR3 Antigen
  • Histocompatibility Antigens Class II
  • invariant chain