Impaired Ca2+ response to glucose in mouse beta-cells infected with coxsackie B or Echo virus

Virus Res. 1994 Sep;33(3):229-40. doi: 10.1016/0168-1702(94)90105-8.


Five strains of Coxsackie B4 virus and one of Echo 11 virus were tested with regard to their ability to replicate in pancreatic mouse beta-cells and interfere with the alterations of the cytoplasmic Ca2+ concentration ([Ca2+]i) induced by glucose. All strains except one both multiplied and caused cytopathic effect. In a control group 68% of the beta-cells responded to 11 mM glucose with large amplitude oscillations of [Ca2+]i. After inoculation with the infectious strains these oscillations appeared in only 5% of the beta-cells, whereas the non-infectious strain did not modify the glucose effect on [Ca2+]i. Despite the virus interference with the glucose response, [Ca2+]i was increased after depolarization with excessive extracellular K+ and the oscillations were induced in most beta-cells when glucose was combined with the insulin-releasing sulfonylurea tolbutamide.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cells, Cultured
  • Cytopathogenic Effect, Viral
  • Cytoplasm / metabolism
  • Enterovirus B, Human / physiology*
  • Glucose / pharmacology*
  • Humans
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / metabolism
  • Islets of Langerhans / virology*
  • Mice
  • Tolbutamide / pharmacology
  • Virus Replication


  • Tolbutamide
  • Glucose
  • Calcium