Toward cataloguing all rice genes: large-scale sequencing of randomly chosen rice cDNAs from a callus cDNA library

Plant J. 1994 Oct;6(4):615-24. doi: 10.1046/j.1365-313x.1994.6040615.x.

Abstract

A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA, Complementary
  • Enzymes / genetics
  • Gene Expression
  • Gene Library*
  • Genes, Plant*
  • Humans
  • Molecular Sequence Data
  • Oryza / genetics*
  • Plant Proteins / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • Enzymes
  • Plant Proteins

Associated data

  • GENBANK/D15075
  • GENBANK/D15076
  • GENBANK/D15077
  • GENBANK/D15078
  • GENBANK/D15079
  • GENBANK/D15080
  • GENBANK/D15081
  • GENBANK/D15082
  • GENBANK/D15083
  • GENBANK/D15084
  • GENBANK/D15085
  • GENBANK/D15086
  • GENBANK/D15087
  • GENBANK/D15088
  • GENBANK/D15089
  • GENBANK/D15090
  • GENBANK/D15091
  • GENBANK/D15092
  • GENBANK/D15093
  • GENBANK/D15094
  • GENBANK/D15095
  • GENBANK/D15096
  • GENBANK/D15097
  • GENBANK/D15098
  • GENBANK/D15099
  • GENBANK/D15100
  • GENBANK/D15101
  • GENBANK/D15102
  • GENBANK/D15103
  • GENBANK/D15104