Expression of cytochrome P-450 3A in HT29-MTX cells and Caco-2 clone TC7

FEBS Lett. 1994 Dec 5;355(3):247-50. doi: 10.1016/0014-5793(94)01199-0.


HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Blotting, Western
  • Carcinoma / enzymology*
  • Cell Compartmentation
  • Cell Differentiation
  • Clone Cells
  • Colonic Neoplasms / enzymology*
  • Cytochrome P-450 CYP2E1
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Cytochrome P-450 Enzyme System / genetics
  • Drug Resistance / genetics
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Methotrexate / pharmacology
  • Mixed Function Oxygenases / biosynthesis*
  • Mixed Function Oxygenases / genetics
  • RNA, Messenger / analysis
  • Selection, Genetic
  • Tumor Cells, Cultured


  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Cytochrome P-450 CYP2E1
  • Methotrexate