Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

FEBS Lett. 1994 Dec 12;356(1):51-5. doi: 10.1016/0014-5793(94)01231-8.

Abstract

The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Aorta
  • Chickens
  • Cloning, Molecular
  • DNA, Complementary
  • Gizzard, Avian
  • Leucine Zippers / genetics
  • Molecular Sequence Data
  • Muscle, Smooth / enzymology*
  • Phosphoprotein Phosphatases / genetics*
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Phosphoprotein Phosphatases

Associated data

  • GENBANK/S74622
  • GENBANK/S74623
  • GENBANK/S74907