ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase

J Biol Chem. 1994 Dec 16;269(50):31383-9.


Two recessive alleles of ERG10 and three temperature-sensitive recessive alleles of HMG1 (3-hydroxy-3-methyl-glutaryl-CoA reductase isoenzyme 1) were isolated in a screen for mevalonate auxotrophs in Saccharomyces cerevisiae. The essential, single-copy ERG10 gene was cloned by complementation of the temperature-sensitive phenotype of erg10-21. The 1,194-base pair continuous open reading frame, encoding a 398-amino acid polypeptide with a calculated molecular mass of 41,681 daltons, was demonstrated to encode cytoplasmic aceto-acetyl-CoA thiolase. Acetoacetyl-CoA thiolase activity corresponded to the number of copies of ERG10 present in cell extracts, and null alleles of ERG10 produced no detectable acetoacetyl-CoA thiolase enzyme activity. The deduced amino acid sequence was 40-95% identical to acetoacetyl-CoA thiolases from other organisms. This identity included the active site cysteines located at amino acids 91 and 384 in the Erg10 protein.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyl-CoA C-Acetyltransferase / genetics*
  • Alleles
  • Amino Acid Sequence
  • Binding Sites
  • Cloning, Molecular
  • Consensus Sequence
  • Cytoplasm / enzymology
  • DNA, Fungal / genetics
  • Genes, Fungal*
  • Genetic Complementation Test
  • Mevalonic Acid / metabolism
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid


  • DNA, Fungal
  • Acetyl-CoA C-Acetyltransferase
  • Mevalonic Acid

Associated data

  • GENBANK/D00511
  • GENBANK/D13470
  • GENBANK/D90476
  • GENBANK/D90477
  • GENBANK/D90478
  • GENBANK/D90479
  • GENBANK/D90480
  • GENBANK/D90481
  • GENBANK/D90482
  • GENBANK/D90483
  • GENBANK/D90484
  • GENBANK/D90485
  • GENBANK/D90486
  • GENBANK/D90487
  • GENBANK/L01113
  • GENBANK/L20428
  • GENBANK/X07976