Identification of GPCMV infected cells in vitro and in vivo with a monoclonal antibody

J Virol Methods. 1994 Jul;48(2-3):133-44. doi: 10.1016/0166-0934(94)90113-9.

Abstract

A monoclonal antibody was made which identifies a 160-180 kDa structural protein in guinea pig cytomegalovirus (GPCMV) infected cells by Western blot using non-reducing conditions. This protein was shown to be a virion structural protein by purification of GPCMV on a density viscosity gradient and Western blot analysis. Phosphoanacetic acid (PAA) experiments suggest that the protein is a late GPCMV protein. In vitro the monoclonal antibody labels a cytoplasmic protein in infected guinea pig embryo fibroblasts by 12 h postinfection. The monoclonal antibody also identifies GPCMV infected cells in vivo in paraffin embedded formalin fixed tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibodies, Viral
  • Blotting, Western
  • Cytomegalovirus / immunology*
  • Cytomegalovirus / isolation & purification*
  • Cytomegalovirus Infections / diagnosis
  • Cytomegalovirus Infections / virology
  • Evaluation Studies as Topic
  • Guinea Pigs
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Neutralization Tests
  • Salivary Glands / virology
  • Viral Structural Proteins / chemistry
  • Viral Structural Proteins / immunology
  • Virology / methods*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Viral Structural Proteins