Abstract
A monoclonal antibody was made which identifies a 160-180 kDa structural protein in guinea pig cytomegalovirus (GPCMV) infected cells by Western blot using non-reducing conditions. This protein was shown to be a virion structural protein by purification of GPCMV on a density viscosity gradient and Western blot analysis. Phosphoanacetic acid (PAA) experiments suggest that the protein is a late GPCMV protein. In vitro the monoclonal antibody labels a cytoplasmic protein in infected guinea pig embryo fibroblasts by 12 h postinfection. The monoclonal antibody also identifies GPCMV infected cells in vivo in paraffin embedded formalin fixed tissue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal*
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Antibodies, Viral
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Blotting, Western
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Cytomegalovirus / immunology*
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Cytomegalovirus / isolation & purification*
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Cytomegalovirus Infections / diagnosis
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Cytomegalovirus Infections / virology
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Evaluation Studies as Topic
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Guinea Pigs
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Immunohistochemistry
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Mice
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Mice, Inbred BALB C
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Molecular Weight
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Neutralization Tests
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Salivary Glands / virology
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Viral Structural Proteins / chemistry
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Viral Structural Proteins / immunology
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Virology / methods*
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Viral Structural Proteins