A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes

Nature. 1994 Dec 8;372(6506):567-70. doi: 10.1038/372567a0.


Cells of the yeast Saccharomyces cerevisiae choose bud sites in a non-random spatial pattern that depends on mating type: axial for haploid cells and bipolar for a/alpha diploid cells. We identified a mutant yeast, axl 1, in which the budding pattern is altered from axial to bipolar. Expression of the AXL1 gene is repressed in a/alpha diploid cells. With the ectopic expression of AXL1, a/alpha cells exhibited an axial budding pattern, thus AXL1 is a key morphological determinant that distinguishes the budding pattern of haploid cells from that of a/alpha diploid cells. AXL1 encodes a protein similar in sequence of the human and Drosophila insulin-degrading enzymes and to the Escherichia coli ptr gene product. The axial budding pattern might result from degradation of a target protein by the putative Axl1 protease.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Division
  • DNA
  • Drosophila
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology
  • Genes, Fungal
  • Genes, Mating Type, Fungal
  • Humans
  • Insulin / metabolism
  • Metalloendopeptidases
  • Molecular Sequence Data
  • Mutation
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid


  • Fungal Proteins
  • Insulin
  • Saccharomyces cerevisiae Proteins
  • DNA
  • AXL1 protein, S cerevisiae
  • Metalloendopeptidases

Associated data

  • GENBANK/D17787