The inheritance of an extrakaryotic mutation conferring temperature-sensitive growth on non-fermentable substrates and a high frequency of mutation to rho- has been studied. Multifactorial crosses (rho+ X rho+) involving this mutation TS8 and mitochondrial mutations conferring resistance to chloramphenicol, erythromycin, oligomycin or paromomycin revealed: a) Mutation TS8 is localized on the mitDNA, referring to a new gene locus TSM1. b) Locus TSM1 appears to be weakly linked to the locus PAR1 and to the loci RIB1 and RIB3 but unlinked to the locus OLI1. c) The position of TSM1 is between PAR1 and the two closely linked loci RIB1 and RIB3, OLI1 is outside and not linked to the segment PAR-TSM-RIB. d) Mutation TS8 does not significantly influence the process of mitochondrial recombination and its control by the mitochondrial locus omega.