Multiple Group II Self-Splicing Introns in Mobile DNA From Escherichia Coli

C R Acad Sci III. 1994 Feb;317(2):141-8.

Abstract

By PCR (polymerase chain reaction) amplification and cloning, we have identified four group II self-splicing introns encoding proteins related to reverse transcriptases in natural Escherichia coli isolates belonging to the ECOR collection. One intron, IntD, interrupts a DNA sequence virtually identical to that of the previously described IS3411 Insertion Sequence. A second intron, IntC, is located within an open reading frame that is closely related to a reading frame in the T-DNA of Agrobacterium tumefaciens. Finally, introns IntA and IntB are inserted at two distinct sites in one of the Rhs elements of E. coli. A comparison of their open reading frames shows that the two Rhs introns are more closely related to each other than to any other known group II intron: this suggests that transposition of group II introns may occur preferentially in cis, along the same piece of DNA.

MeSH terms

  • DNA Transposable Elements / drug effects*
  • DNA Transposable Elements / genetics
  • Escherichia coli / genetics*
  • In Vitro Techniques
  • Introns / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Catalytic / pharmacology*

Substances

  • DNA Transposable Elements
  • RNA, Catalytic

Associated data

  • GENBANK/X77508