Application of physico-chemical typing methods for the epidemiological analysis of Salmonella enteritidis strains of phage type 25/17

Epidemiol Infect. 1994 Dec;113(3):411-24. doi: 10.1017/s0950268800068424.

Abstract

Eighty-nine Salmonella enteritidis phage type 25/17 strains isolated from a localized outbreak in the German state Nordrhein-Westfalen (outbreak NWI) could not be further differentiated by biochemotyping and plasmid pattern analysis. They were submitted to a complex typing system consisting of modern physico-chemical analytical procedures. In lipopolysaccharide pattern analysis the strains proved to be homogeneous. In multilocus enzyme electrophoresis, outer membrane and whole cell protein pattern (WCPP) analysis, and Fourier-transform infrared (FT-IR) spectroscopy (increasing extent of differentiation in the given order) strains deviating from each basal pattern were found. The extent of correspondence in these deviations was satisfactory. Forty-six strains of the same sero- and phage type, however, obtained from different outbreaks, were additionally typed. The results obtained with them indicate that the data of the first group were not restricted to strains from outbreak NWI, but of general validity. It was found that both WCPP and FT-IR represent valuable methods for the sub-grouping of bacteria.

MeSH terms

  • Bacterial Outer Membrane Proteins / analysis
  • Bacterial Proteins / analysis
  • Bacterial Typing Techniques*
  • Bacteriophage Typing
  • Disease Outbreaks*
  • Electrophoresis, Starch Gel
  • Enzymes / analysis
  • Fatty Acids / analysis
  • Germany / epidemiology
  • Humans
  • Lipopolysaccharides / analysis
  • Plasmids
  • Salmonella Infections / epidemiology*
  • Salmonella Infections / microbiology
  • Salmonella enteritidis / chemistry
  • Salmonella enteritidis / classification*
  • Salmonella enteritidis / genetics
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Enzymes
  • Fatty Acids
  • Lipopolysaccharides