Increased binding activity of measles virus to monkey red blood cells after long-term passage in Vero cell cultures

J Gen Virol. 1994 Dec;75 ( Pt 12):3511-6. doi: 10.1099/0022-1317-75-12-3511.

Abstract

Recent field isolates of measles virus (MV) obtained by using B95-8 cells have been reported not to agglutinate African green monkey red blood cells (AGM-RBC). Vero cell-adapted, plaque-forming strains derived from three field isolates at the third passage in Vero cell cultures (T8Ve-3, T11Ve-3 and N13Ve-3) also exhibited markedly decreased binding activity, as determined by infectivity-absorption and haemadsorption tests. On the other hand, binding activity of the respective strains at the twentieth passage (T8Ve-20, T11Ve-20 and N13Ve-20) increased to practically the same level as that of the Edmonston strain, a standard strain of MV passaged long-term. A membrane immunofluorescence test revealed that the decreased binding activity to AGM-RBC of T8Ve-3, T11Ve-3 and N13Ve-3 was not due to decreased expression of the haemagglutinin (H) protein on the cell surface. The deduced amino acid sequence of the H protein synthesized in T11Ve-3-infected cells was identical to that in T11Ve-20-infected cells, although a single amino acid alteration was observed when T8Ve-3 was compared with T8Ve-20. Similarly, approximately half of the N13Ve-20-infected cells synthesized an H protein identical to that produced in N13Ve-3-infected cells, and nevertheless, exhibited markedly increased haemadsorption. The present results suggest that a viral protein(s) other than the H protein contributed to the binding activity of MV to AGM-RBC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Membrane / metabolism
  • Chlorocebus aethiops
  • Erythrocytes / metabolism*
  • Erythrocytes / physiology
  • Genetic Variation / genetics
  • Hemagglutination, Viral / physiology*
  • Hemagglutinins, Viral / biosynthesis
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / metabolism*
  • Humans
  • Measles virus / growth & development
  • Measles virus / metabolism*
  • Molecular Sequence Data
  • Serial Passage
  • Vero Cells / virology
  • Viral Plaque Assay
  • Viral Proteins / metabolism

Substances

  • Hemagglutinins, Viral
  • Viral Proteins
  • hemagglutinin protein G, measles virus

Associated data

  • GENBANK/D28942
  • GENBANK/D28943
  • GENBANK/D28944
  • GENBANK/D28945
  • GENBANK/D28946
  • GENBANK/D28947
  • GENBANK/D28948
  • GENBANK/D28949
  • GENBANK/D28950
  • GENBANK/D28951