T cells from children with IDDM are sensitized to bovine serum albumin

Scand J Immunol. 1994 Dec;40(6):623-8. doi: 10.1111/j.1365-3083.1994.tb03514.x.

Abstract

Epidemiological and experimental evidence suggested that denial of dietary cow milk protein early in life protects genetically susceptible children and animals from insulin-dependent diabetes (IDDM). Bovine serum albumin (BSA) was proposed as a candidate milk-borne mimicry antigen responsible for the diabetogenic cow milk effect. Elevated anti-BSA antibodies have been observed in patients and diabetic rodents, and these antibodies precipitate p69 from islet cell lysates. IDDM is a T cell mediated disorder but efforts to detect BSA-specific T cells in diabetic children have so far failed. We describe here a culture system which allowed the detection of BSA-specific T cells and we mapped this response to the ABBOS peptide (pre-BSA position 152-169) previously identified as a possible mimicry epitope. ABBOS-sensitized T cells were found in 28/31 children with recent onset IDDM but not in non-diabetic controls nor in children with SLE or JRA. T cell proliferative responses declined within the first few years of diabetes diagnosis. Although no effector cell role for BSA/ABBOS specific T lymphocytes has been demonstrated, the presence of BSA peptide-specific T cells strengthens the postulated link between a cow milk protein and IDDM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Amino Acid Sequence
  • Child
  • Diabetes Mellitus, Type 1 / immunology*
  • Female
  • Humans
  • Male
  • Molecular Sequence Data
  • Peptide Fragments / immunology
  • Serum Albumin, Bovine / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Peptide Fragments
  • Serum Albumin, Bovine