Molecular cloning of rat RAC protein kinase alpha and beta and their association with protein kinase C zeta

Biochem Biophys Res Commun. 1994 Nov 30;205(1):817-25. doi: 10.1006/bbrc.1994.2738.

Abstract

cDNAs of RAC protein kinases (RAC-PK) alpha and beta were cloned from a rat testis cDNA library. The predicted open reading frames encode 480 and 481 amino acids of RAC-PK alpha and beta, respectively, and the rat RAC-PK alpha and beta have sequences conserved among different mammalian species such as the pleckstrin homology domain at their amino-terminal region and the protein-serine/threonine kinase catalytic domain at their carboxyl-terminal region. RNA blot analysis showed wide distribution of two RAC-PK in rat tissues. Immunoprecipitation analysis revealed that RAC-PK alpha and beta associate with protein kinase C zeta through the pleckstrin homology domain in vitro, suggesting the interaction between RAC-PK and protein kinase C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Isoenzymes / metabolism*
  • Molecular Sequence Data
  • Protein Kinase C / metabolism*
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism
  • Proto-Oncogene Proteins c-akt
  • Rats
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • DNA, Complementary
  • Isoenzymes
  • protein kinase C gamma
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt
  • Protein Kinase C

Associated data

  • GENBANK/D30040
  • GENBANK/D30041