Conjugation Reactions of Cyclodepsipeptide to Glutathionyl Adducts by Direct 'In-Beam' Synthesis Under Negative-Ion Fast-Atom Bombardment Conditions

Rapid Commun Mass Spectrom. 1994 Oct;8(10):844-8. doi: 10.1002/rcm.1290081010.

Abstract

The conjugation products of E-destruxin (cyclodepsipeptide) with glutathione have been identified by fast-atom bombardment mass spectrometry. Appropriate conditions were developed for cyclodepsipeptide substrate, which enabled the direct, dynamic mass spectra analysis of spontaneous as well as glutathione-s-transferase catalyzed conjugation reactions. Application to the most active cyclopeptide in the series of destruxins yielded glutathionyl adduct.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Depsipeptides*
  • Fungal Proteins*
  • Glutathione / chemistry*
  • Glutathione Transferase / metabolism
  • Mitosporic Fungi / chemistry
  • Molecular Sequence Data
  • Mycotoxins / chemistry*
  • Peptides, Cyclic / chemistry*
  • Spectrometry, Mass, Fast Atom Bombardment

Substances

  • Depsipeptides
  • Fungal Proteins
  • Mycotoxins
  • Peptides, Cyclic
  • Glutathione Transferase
  • Glutathione
  • destruxin E