Constitutive expression of the Id1 gene impairs mouse B cell development

Cell. 1994 Dec 2;79(5):893-900. doi: 10.1016/0092-8674(94)90078-7.


The Id proteins are inhibitors of the basic-helix-loop-helix (bHLH) transcription factors. In the B cell lineage, the Id1 and Id2 genes are expressed in pro-B cells and down-regulated during differentiation. To determine the role of bHLH proteins and the significance of down-regulation of Id genes in B cell development, transgenic mice constitutively expressing the Id1 gene were generated. Their phenotype suggests that B cell development is impaired at an early stage. Primarily, these mice have few B220+ IgM+ mature B or B220+ CD43- pre-B cells in the bone marrow, reduced frequencies of V(D)J and V kappa J kappa recombination of the immunoglobulin loci, and lower expression levels of the immunoglobulin, RAG-1, RAG-2, and lambda 5 genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / physiology*
  • Cell Differentiation
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Dosage
  • Gene Expression Regulation
  • Genes, Immunoglobulin / genetics
  • Helix-Loop-Helix Motifs*
  • Inhibitor of Differentiation Protein 1
  • Mice
  • Mice, Transgenic
  • Recombination, Genetic
  • Repressor Proteins*
  • TCF Transcription Factors
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • Idb1 protein, mouse
  • Inhibitor of Differentiation Protein 1
  • Repressor Proteins
  • TCF Transcription Factors
  • Tcf7l1 protein, mouse
  • Transcription Factor 7-Like 1 Protein
  • Transcription Factors