Abstract
KIN28, a member of the p34cdc2/CDC28 family of protein kinases, is identified as a subunit of yeast RNA polymerase transcription factor IIH (TFIIH) on the basis of sequence determination, immunological reactivity, and copurification. KIN28 is, moreover, one of three subunits of TFIIK, a subassembly of TFIIH with protein kinase activity directed toward the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II. Itself a phosphoprotein, KIN28 interacts specifically with the two largest subunits of RNA polymerase II. Previous work of others points to two further associations: KIN28 interacts in vivo with the cyclin CCL1, and KIN28 and CCL1 are homologous to human MO15 and cyclin H, which form the cyclin-dependent kinase-activating kinase (CAK). We show that human CAK possesses the CTD kinase activity characteristic of TFIIH.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cell-Free System
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Cyclin-Dependent Kinase-Activating Kinase
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Cyclin-Dependent Kinases*
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Cyclins / metabolism
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Humans
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Molecular Sequence Data
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Phosphoric Monoester Hydrolases / metabolism
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Protein Binding
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Protein Biosynthesis
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Protein Serine-Threonine Kinases / genetics*
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Protein Serine-Threonine Kinases / immunology
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Protein Serine-Threonine Kinases / metabolism
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RNA Polymerase II / metabolism*
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis
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Sequence Homology, Amino Acid
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TATA-Binding Protein Associated Factors*
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Transcription Factor TFIID*
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Transcription Factor TFIIH
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription Factors, TFII*
Substances
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Cyclins
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Recombinant Proteins
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Saccharomyces cerevisiae Proteins
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TAF6 protein, S cerevisiae
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TATA-Binding Protein Associated Factors
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Transcription Factor TFIID
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Transcription Factors
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Transcription Factors, TFII
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Transcription Factor TFIIH
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Protein Serine-Threonine Kinases
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Cyclin-Dependent Kinases
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Kin28 protein kinase, S cerevisiae
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RNA Polymerase II
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Phosphoric Monoester Hydrolases
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Cyclin-Dependent Kinase-Activating Kinase
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CDK7 protein, human