Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK

Cell. 1994 Dec 16;79(6):1103-9. doi: 10.1016/0092-8674(94)90040-x.


KIN28, a member of the p34cdc2/CDC28 family of protein kinases, is identified as a subunit of yeast RNA polymerase transcription factor IIH (TFIIH) on the basis of sequence determination, immunological reactivity, and copurification. KIN28 is, moreover, one of three subunits of TFIIK, a subassembly of TFIIH with protein kinase activity directed toward the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II. Itself a phosphoprotein, KIN28 interacts specifically with the two largest subunits of RNA polymerase II. Previous work of others points to two further associations: KIN28 interacts in vivo with the cyclin CCL1, and KIN28 and CCL1 are homologous to human MO15 and cyclin H, which form the cyclin-dependent kinase-activating kinase (CAK). We show that human CAK possesses the CTD kinase activity characteristic of TFIIH.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cell-Free System
  • Cyclin-Dependent Kinases*
  • Cyclins / metabolism
  • Humans
  • Molecular Sequence Data
  • Phosphoric Monoester Hydrolases / metabolism
  • Protein Binding
  • Protein Biosynthesis
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / immunology
  • Protein-Serine-Threonine Kinases / metabolism
  • RNA Polymerase II / metabolism*
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • TATA-Binding Protein Associated Factors*
  • Transcription Factor TFIID*
  • Transcription Factor TFIIH
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcription Factors, TFII*


  • Cyclins
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • TAF6 protein, S cerevisiae
  • TATA-Binding Protein Associated Factors
  • Transcription Factor TFIID
  • Transcription Factors
  • Transcription Factors, TFII
  • Transcription Factor TFIIH
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • cyclin-dependent kinase-activating kinase
  • Kin28 protein kinase, S cerevisiae
  • RNA Polymerase II
  • Phosphoric Monoester Hydrolases