Optimized conditions for the fermentation of Helicobacter pylori and production of vacuolating cytotoxin

FEMS Microbiol Lett. 1994 Nov 15;124(1):55-9. doi: 10.1111/j.1574-6968.1994.tb07261.x.


We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvement in the production of vacuolating cytotoxin.

MeSH terms

  • Bacterial Proteins / biosynthesis*
  • Bacterial Toxins / biosynthesis
  • Biomass
  • Culture Media*
  • Cytotoxins / biosynthesis*
  • Fermentation
  • Glucose
  • Helicobacter pylori / growth & development
  • Helicobacter pylori / metabolism*


  • Bacterial Proteins
  • Bacterial Toxins
  • Culture Media
  • Cytotoxins
  • VacA protein, Helicobacter pylori
  • Glucose