Expression of TGF-beta s and TGF-beta type II receptor mRNAs in mouse folliculogenesis: stored maternal TGF-beta 2 message in oocytes

Biochem Biophys Res Commun. 1994 Jun 15;201(2):649-56. doi: 10.1006/bbrc.1994.1750.

Abstract

The present in situ hybridisation study describes expression of TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta type II receptor mRNA during follicular development, and the temporal pattern and abundance of TGF-beta 2 transcripts in early pre-implantation embryos. TGF-beta 1 hybridisation signals were most prominent in the outer granulosa cell layers of the post-antral follicles and in the corpus luteum, whereas strong expression of TGF-beta type II receptor mRNA was confined to thecal cells. Weak TGF-beta 3 mRNA expression was observed in all major cell types of pre- and post-antral follicles. Most notably, the very strong TGF-beta 2 hybridisation signals which were detected in developing oocytes declined rapidly following fertilization. Although still abundant in two-cell embryos, TGF-beta 2 hybridisation signals were barely detectable in four- and eight-cell embryos. These findings, and the presence of specific sequence motifs in the 3' untranslated region of TGF-beta 2 mRNAs, suggest that TGF-beta 2 transcripts are stored as maternal messages.

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Corpus Luteum / metabolism
  • Estrus
  • Female
  • Gene Expression*
  • Granulosa Cells / metabolism
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Oocytes / metabolism*
  • Ovarian Follicle / cytology
  • Ovarian Follicle / metabolism*
  • Ovary / cytology
  • Ovary / metabolism*
  • Protein-Serine-Threonine Kinases
  • RNA Probes
  • RNA, Messenger / biosynthesis
  • Receptor, Transforming Growth Factor-beta Type II
  • Receptors, Transforming Growth Factor beta / biosynthesis*
  • Superovulation
  • Theca Cells / metabolism
  • Transforming Growth Factor beta / biosynthesis*

Substances

  • RNA Probes
  • RNA, Messenger
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta
  • Protein-Serine-Threonine Kinases
  • Receptor, Transforming Growth Factor-beta Type II