Interferon-gamma Induces Resistance in Primary Monocytes Against Human Immunodeficiency Virus type-1 Infection

Biochem Biophys Res Commun. 1994 Jun 15;201(2):756-61. doi: 10.1006/bbrc.1994.1765.

Abstract

Treatment of monocytes with interferon-gamma 1 day before, or at the time of infection with human immunodeficiency virus type-1 (HIV-1) induced complete resistance in monocytes against HIV-1 infection. There was no evidence of viral RNA, proviral DNA, p24 antigen, or reverse transcriptase activity through 2 weeks after inoculation. Ultrastructural examination of these cells showed no detectable virus particles. When interferon-gamma was added to monocytes 1 to 3 days post-infection, virus integration occurred, but the viral expression was either ablated (1 day post-infection) or significantly inhibited (3 days post-infection). Treatment of monocytes with interferon-gamma before or after infection with HIV-1 produced significantly higher levels of tumor necrosis factor-alpha and interleukin-8 than untreated or uninfected monocytes. These results suggest that altered regulation of cytokines may mediate antiviral activity of interferon-gamma in monocytes.

MeSH terms

  • Cells, Cultured
  • DNA, Viral / analysis*
  • Gene Expression Regulation, Viral / drug effects
  • Genes, gag
  • HIV-1 / isolation & purification
  • HIV-1 / physiology*
  • HIV-1 / ultrastructure
  • Humans
  • Interferon-gamma / pharmacology*
  • Interleukin-8 / biosynthesis
  • Leukapheresis
  • Microscopy, Electron
  • Monocytes / drug effects
  • Monocytes / immunology*
  • Monocytes / microbiology
  • Polymerase Chain Reaction / methods
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Virus Integration / drug effects
  • Virus Integration / physiology*

Substances

  • DNA, Viral
  • Interleukin-8
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma