Differential expression of two major cytokines produced by neutrophils, interleukin-8 and the interleukin-1 receptor antagonist, in neutrophils isolated from the synovial fluid and peripheral blood of patients with rheumatoid arthritis

Arthritis Rheum. 1994 Jun;37(6):855-9. doi: 10.1002/art.1780370613.


Objective: Neutrophils have been shown to produce interleukin-8 (IL-8) and interleukin-1 receptor antagonist (IL-1ra) in large amounts compared with other cytokines. Since IL-8 has a proinflammatory action whereas IL-1ra is antiinflammatory, our objective was to examine the relative levels of production of these cytokines by synovial fluid (SF) neutrophils isolated from patients with rheumatoid arthritis (RA).

Methods: We measured cytokine production using an enzyme-linked immunosorbent assay and analyzed messenger RNA accumulation in cells by Northern blot.

Results: SF neutrophils produced significantly more IL-8 and IL-1 beta, but significantly less IL-1ra, than peripheral blood neutrophils.

Conclusion: These observations provide new information on the production of pro- and antiinflammatory molecules by neutrophils in the SF environment, and their possible role in RA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Rheumatoid* / blood
  • Arthritis, Rheumatoid* / pathology
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / analysis
  • Interleukin-8 / analysis*
  • Interleukin-8 / genetics
  • Neutrophils / chemistry*
  • RNA, Messenger / analysis
  • Sialoglycoproteins / analysis*
  • Sialoglycoproteins / genetics
  • Synovial Fluid* / cytology


  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Interleukin-8
  • RNA, Messenger
  • Sialoglycoproteins