Frozen-thawed cumulus-granulosa cells support bovine embryo development during coculture

Fertil Steril. 1994 Jul;62(1):176-80.


Objective: To evaluate the ability of bovine cumulus-granulosa cells to survive cryopreservation and subsequently support bovine embryo development during coculture.

Design: In vitro-matured and -fertilized bovine embryos (two- to four-cell) were allotted randomly to one of three treatment groups: [1] control medium alone consisting of Medium 199 containing 10% fetal bovine serum and antibiotics, [2] cocultured on fresh bovine cumulus-granulosa cells in control medium, or [3] cocultured on frozen-thawed cumulus-granulosa cells in control medium. Embryo development was assessed on days 7 and 8 after IVF.

Results: Coculture improved embryo development on days 7 and 8 compared with the control group. However, embryo development on days 7 and 8 did not differ among coculture groups.

Conclusions: Frozen-thawed cumulus-granulosa cells enhance embryo development similar to fresh cells during in vitro coculture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle / embryology*
  • Culture Techniques / methods
  • Embryonic and Fetal Development*
  • Female
  • Freezing*
  • Granulosa Cells* / physiology*
  • Ovarian Follicle / cytology*