Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe

Genetics. 1994 Mar;136(3):849-56.

Abstract

Homologous integration into the fission yeast Schizosaccharomyces pombe has not been well characterized. In this study, we have examined integration of plasmids carrying the leu1+ and ura4+ genes into their chromosomal loci. Genomic DNA blot analysis demonstrated that the majority of transformants have one or more copies of the plasmid vector integrated via homologous recombination with a much smaller fraction of gene conversion to leu1+ or ura4+. Non-homologous recombination events were not observed for either gene. We describe the construction of generally useful leu1+ and ura4+ plasmids for targeted integration at the leu1-32 and ura4-294 loci of S. pombe.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • DNA, Fungal / genetics
  • Gene Conversion
  • Genes, Fungal*
  • Genetic Vectors
  • Recombination, Genetic
  • Schizosaccharomyces / genetics*
  • Transformation, Genetic*

Substances

  • DNA, Fungal