Correlation between the BANA test and oral malodor parameters

J Dent Res. 1994 May;73(5):1036-42. doi: 10.1177/00220345940730050401.


The purpose of the present investigation was to test the association between the BANA test (Perioscan, Oral-B), and oral malodor parameters. The subject population consisted of 52 Israeli adults, 43 of whom complained of oral malodor. Oral malodor measurements consisted of peak and steady-state volatile sulphide measurement by a portable sulphide monitor (Interscan Corp., model 1170), as well as organoleptic measurements of malodor from whole mouth, tongue, and saliva. Samples for the BANA test were obtained from four loci (shallow pocket, deep pocket, tongue dorsum, saliva); results were scored as negative (0), weak (1), or strong (2). BANA scores were significantly associated with odor-judge ratings, with the highest association obtained when BANA saliva scores and odor-judge saliva assessment were compared (r = 0.500; p < 0.001). BANA tests from the different loci were not significantly associated with sulphide monitor levels. Stepwise multiple-regression analysis of odor-judge measurements in terms of sulphide levels and average BANA scores showed that both log peak sulphide levels as well as BANA scores were significantly factored into the equations, yielding, in all cases, highly significant correlations (multiple r = 0.57, 0.50, and 0.59, respectively, with significance levels of 0.0001, 0.001, and < 0.0001, for whole mouth, tongue, and saliva malodor, respectively). The results suggest that the BANA scores are associated with a component of oral malodor which is independent of volatile sulphide measurements and suggest its use as an adjunct test to volatile sulphide measurement.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Analysis of Variance
  • Bacteroides / enzymology
  • Benzoylarginine-2-Naphthylamide*
  • Child
  • Clinical Enzyme Tests*
  • Dental Plaque / microbiology
  • Female
  • Halitosis / diagnosis*
  • Halitosis / enzymology
  • Halitosis / microbiology
  • Humans
  • Hydrogen Sulfide / analysis
  • Hydrogen Sulfide / metabolism
  • Middle Aged
  • Periodontal Pocket / microbiology*
  • Porphyromonas gingivalis / enzymology
  • Regression Analysis
  • Saliva / microbiology
  • Sulfhydryl Compounds / analysis*
  • Sulfhydryl Compounds / metabolism
  • Tongue / microbiology
  • Treponema / enzymology


  • Sulfhydryl Compounds
  • methylmercaptan
  • Benzoylarginine-2-Naphthylamide
  • Hydrogen Sulfide