Negative feedback regulation of IgE synthesis by murine CD23

Nature. 1994 Jun 30;369(6483):753-6. doi: 10.1038/369753a0.


Immunoglobulin E is found in nanogram amounts in normal human and mouse serum. It is increased during parasitic infestations and mediates allergy. CD23, the low-affinity receptor for IgE (Fc epsilon RII), has been proposed as an important regulator of IgE synthesis. The type-II transmembrane lectin CD23 is expressed in the mouse on B cells and follicular dendritic cells. In humans there are two forms of CD23 which differ in their intracellular amino-terminal 6/7 amino acids; expression of the A-form corresponds to that of murine CD23, whereas the B-form is also found on T and other haematopoietic cells. CD23 has been implicated in cellular adhesion, antigen presentation, as a growth and differentiation factor for human B, T and plasma cells, and as a signal transduction molecule (reviewed in refs 3, 8). Here we disrupt the gene coding for murine CD23 (ref. 9) to clarify the role of CD23 in vivo and find that B- and T-cell development is normal in these CD23-deficient mice. Immune responses to the helminth Nippostrongylus brasiliensis are unaffected. In contrast, immunization with thymus-dependent antigens leads to increased and sustained specific IgE antibody titres compared with controls. Formation of germinal centres is normal. These results suggest that murine CD23 acts as a negative feedback component of IgE regulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • Bone Marrow / immunology
  • Bone Marrow Cells
  • Cell Differentiation
  • Cell Line
  • Dinitrophenols / immunology
  • Feedback
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin E / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mutagenesis
  • Nippostrongylus / immunology
  • Ovalbumin / immunology
  • Receptors, IgE / genetics
  • Receptors, IgE / physiology*
  • T-Lymphocytes / cytology
  • Thymus Gland / cytology
  • Thymus Gland / immunology


  • Dinitrophenols
  • Receptors, IgE
  • dinitrophenol-ovalbumin
  • Immunoglobulin E
  • Ovalbumin