Effects of aldehyde-modified proteins on mesangial cell-matrix interaction

Diabetes Res Clin Pract. 1994 Feb;23(1):25-32. doi: 10.1016/0168-8227(94)90123-6.


We examined the effect of aldehyde-modified matrix macromolecules on mesangial cell function in vitro, using incubated rat glomerular mesangial cells. Laminin and fibronectin were modified by incubation for 24 h with 50 mM glycolaldehyde (GA), a highly reactive cross-linking glycation product, with or without equimolar aminoguanidine. GA-modified laminin and fibronectin caused marked inhibition of cell adhesion. Cell spreading was reduced on the GA-modified laminin. In contrast, the GA-modified fibronectin had no effect on cell spreading. The study of thymidine incorporation by mesangial cells showed that the GA-modified fibronectin had a diminished mitogenic activity against cells. The content of advanced glycation end-product (AGE), which was determined by fluorescence at 370 nm excitation and 440 nm emission wavelength, increased and intermolecular cross-links appeared in the GA-modified proteins. To a large extent, aminoguanidine restored the structural alterations and functional deteriorations described above. We conclude that GA-modified matrix proteins diminish their functional properties against mesangial cells and affect cellular functions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetaldehyde / analogs & derivatives*
  • Acetaldehyde / pharmacology
  • Animals
  • Cell Adhesion / drug effects*
  • Cell Division / drug effects
  • Cells, Cultured
  • Extracellular Matrix / physiology
  • Fibronectins / drug effects
  • Fibronectins / metabolism
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / drug effects
  • Glomerular Mesangium / physiology*
  • Glycation End Products, Advanced
  • Guanidines / pharmacology*
  • Laminin / drug effects
  • Laminin / metabolism
  • Male
  • Rats
  • Rats, Wistar
  • Thymidine / metabolism


  • Fibronectins
  • Glycation End Products, Advanced
  • Guanidines
  • Laminin
  • Acetaldehyde
  • pimagedine
  • Thymidine
  • glycolaldehyde