L-arginine-nitric oxide pathway and chronic nephropathy in aged rats

J Gerontol. 1994 Jul;49(4):B157-61. doi: 10.1093/geronj/49.4.b157.

Abstract

Effects of aging and dietary protein on the L-arginine-nitric oxide (Arg-NO) pathway and the progress of chronic nephropathy were examined. At 6-7 months of age, 10 male Fischer 344 rats were fed a 23% protein diet until 24 or 25 months of age, and another 10 were fed a 12% protein diet until that age. Twenty male Fischer 344 rats that were fed the 23% protein diet from 6 to 8 months of age were used as a control. Urinary excretion of nitrite/nitrate (NOx) at the age of 24 months in the 23% protein group was remarkably decreased, whereas in the 12% protein group, urinary NOx remained comparable to that of the control. Histological examination revealed that chronic nephropathy was highly progressive in the 23% protein group, accompanied by lowered renal function, but these changes were obviously suppressed in the 12% protein group. These results suggest that an age-related decrease in the synthesis of NO could be associated with the progress of chronic nephropathy.

MeSH terms

  • Aging / metabolism*
  • Amino Acids / blood
  • Animals
  • Arginine / metabolism*
  • Blood Urea Nitrogen
  • Chronic Disease
  • Creatinine / urine
  • Dietary Proteins / administration & dosage
  • Dietary Proteins / pharmacology
  • Dilatation, Pathologic / pathology
  • Guanidines / urine
  • Kidney Diseases / metabolism*
  • Kidney Diseases / pathology
  • Kidney Glomerulus / pathology
  • Kidney Tubules / pathology
  • Male
  • Nitrates / urine
  • Nitric Oxide / metabolism*
  • Nitrites / urine
  • Rats
  • Rats, Inbred F344
  • Sclerosis
  • Specific Pathogen-Free Organisms

Substances

  • Amino Acids
  • Dietary Proteins
  • Guanidines
  • Nitrates
  • Nitrites
  • Nitric Oxide
  • Arginine
  • Creatinine