Bombesin, gastrin-releasing peptide, and neuromedin C modulate murine lymphocyte proliferation through adherent accessory cells and activate protein kinase C

Peptides. 1994 Jan;15(1):15-22. doi: 10.1016/0196-9781(94)90164-3.


Recent data have shown the ability of bombesin-related peptides to stimulate murine macrophage functions. In the present study, we have investigated the effect of bombesin, gastrin-releasing peptide (GRP), and neuromedin C on the proliferative response of lymphocytes from murine axillary nodes, spleen, and thymus. The results show that these neuropeptides at 10(-9), 10(-10), and 10(-11) M concentrations modulate the lymphoproliferative response, stimulating to a small but significant extent the spontaneous proliferation and inhibiting to a great extent the lymphoproliferative response to the mitogen concanavalin A (Con A). This regulation is probably mediated through adherent accessory cells, since their presence for the neuropeptides to produce their effect. The increased interleukin-1 beta production by Con A in cultures of peritoneal macrophages (a model of adherent accessory cells) decreased after the addition of bombesin, GRP, and neuromedin C; this diminution is a possible mechanism for their inhibitory action on the lymphoproliferative response to Con A. In addition, these neuropeptides caused a significant protein kinase C activation in total leukocyte population and T-enriched lymphocytes from axillary nodes, as well as in peritoneal macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Presenting Cells / drug effects*
  • Bombesin / pharmacology
  • Cell Adhesion / drug effects
  • Cell Division / drug effects
  • Enzyme Activation
  • Gastrin-Releasing Peptide
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Lymphocytes / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neuropeptides / pharmacology*
  • Peptide Fragments / pharmacology
  • Peptides / pharmacology
  • Protein Kinase C / drug effects*


  • Neuropeptides
  • Peptide Fragments
  • Peptides
  • Gastrin-Releasing Peptide
  • neuromedin C
  • Protein Kinase C
  • Bombesin