New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus

Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5977-81. doi: 10.1073/pnas.91.13.5977.

Abstract

Glc3Man9GlcNAc2 is the preferred substrate of the oligosaccharyltransferase of N-linked glycosylation of proteins, but nonglucosylated oligosaccharides can be transferred to proteins in Saccharomyces cerevisiae. Mutations affecting the addition of the three terminal glucose residues lead to accumulation of Man9GlcNAc2 or Glc1Man9GlcNAc2 in vivo but do not show any detectable growth defect. When these mutations were introduced into a strain with reduced oligosaccharyltransferase activity (due to the wbp1-1 mutation), a severe growth defect was observed: accumulation of suboptimal lipid-linked oligosaccharide and reduced oligosaccharyltransferase activity resulted in a severe underglycosylation of secreted proteins. This new synthetic phenotype made it possible to isolate the ALG8 locus, encoding a potential glucosyltransferase of the endoplasmic reticulum. The ALG8 protein is a 63.5-kDa hydrophobic protein that is not essential for the vegetative growth of yeast. However, the lack of this protein resulted in underglycosylation of secreted proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Carbohydrate Sequence
  • Cloning, Molecular
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics*
  • Genes, Fungal
  • Glucosyltransferases / biosynthesis
  • Glucosyltransferases / genetics*
  • Glycosylation
  • Hexosyltransferases*
  • Lipopolysaccharides / metabolism*
  • Membrane Proteins*
  • Molecular Sequence Data
  • Mutation*
  • Oligosaccharides / metabolism
  • Open Reading Frames
  • Phenotype
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Substrate Specificity
  • Transferases / biosynthesis*
  • Transferases / genetics

Substances

  • Fungal Proteins
  • Lipopolysaccharides
  • Membrane Proteins
  • Oligosaccharides
  • Saccharomyces cerevisiae Proteins
  • lipid-linked oligosaccharides
  • Transferases
  • Alg8 protein, S cerevisiae
  • Glucosyltransferases
  • Hexosyltransferases
  • dolichyl-diphosphooligosaccharide - protein glycotransferase

Associated data

  • GENBANK/X75929