Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting

Science. 1994 Jul 1;265(5168):103-6. doi: 10.1126/science.8016642.


Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Nucleotidyltransferases / genetics
  • DNA Nucleotidyltransferases / metabolism
  • DNA Polymerase I / genetics*
  • DNA Polymerase I / metabolism
  • Female
  • Gene Deletion*
  • Genetic Engineering / methods*
  • Homozygote
  • Integrases*
  • Male
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Mutation
  • Recombination, Genetic
  • Stem Cells / enzymology
  • T-Lymphocytes / enzymology*
  • Transfection
  • Viral Proteins*


  • Viral Proteins
  • Cre recombinase
  • DNA Nucleotidyltransferases
  • Integrases
  • DNA Polymerase I