A Sensitive Reporter Cell Line for HIV-1 Tat Activity, HIV-1 Inhibitors, and T Cell Activation Effects

AIDS Res Hum Retroviruses. 1994 Mar;10(3):295-301. doi: 10.1089/aid.1994.10.295.

Abstract

The production and characterization of a cell line for quantitative HIV-1 Tat function and T cell activation assays is described. 1G5 is a clonal cell line derived from Jurkat T cells stably transfected with a luciferase gene driven by an HIV-1 long terminal repeat (HIV-LTR). The 1G5 clone was selected for low basal luciferase activity, susceptibility to HIV infection, and high responsiveness to Tat and T cell activation signals. A 10 to 1000-fold increase in luciferase activity after transfection or infection with tat-expressing vectors or HIV was observed. Equivalent levels of expression were detected after stimulation with T cell mitogens. The characteristics of 1G5 make it a valuable reagent for studies of HIV infection, HIV regulatory agents, and other T cell or HIV-activating factors, and for screening potential anti-HIV therapeutic agents.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Line*
  • DNA Primers
  • Gene Products, tat / metabolism*
  • Genes, tat
  • HIV-1 / drug effects
  • HIV-1 / physiology*
  • Humans
  • Luciferases / genetics
  • Luciferases / metabolism
  • Lymphocyte Activation
  • Molecular Sequence Data
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / immunology
  • T-Lymphocytes / microbiology*
  • Zidovudine / pharmacology
  • tat Gene Products, Human Immunodeficiency Virus

Substances

  • DNA Primers
  • Gene Products, tat
  • tat Gene Products, Human Immunodeficiency Virus
  • Zidovudine
  • Luciferases