A general enhancement scheme in heteronuclear multidimensional NMR employing pulsed field gradients

J Biomol NMR. 1994 Mar;4(2):301-6. doi: 10.1007/BF00175254.


General pulse sequence elements that achieve sensitivity-enhanced coherence transfer from a heteronucleus to protons of arbitrary multiplicity are introduced. The building blocks are derived from the sensitivity-enhancement scheme introduced by Cavanagh et al. ((1991) J. Magn. Reson., 91, 429-436), which was used in conjunction with gradient coherence selection by Kay et al. ((1992) J. Am. Chem. Soc., 114, 10663-10665), as well as from a multiple-pulse sequence effecting a heteronuclear planar coupling Hamiltonian. The building blocks are incorporated into heteronuclear correlation experiments, in conjunction with coherence selection by the formation of a heteronuclear gradient echo. This allows for efficient water suppression without the need for water presaturation. The methods are demonstrated in HSQC-type experiments on a sample of a decapeptide in H2O. The novel pulse sequence elements can be incorporated into multidimensional experiments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Gonadotropin-Releasing Hormone / chemistry*
  • Indicators and Reagents
  • Magnetic Resonance Spectroscopy / methods*
  • Mathematics
  • Molecular Sequence Data
  • Oligopeptides / chemistry*
  • Protein Conformation*
  • Sensitivity and Specificity


  • Indicators and Reagents
  • Oligopeptides
  • Gonadotropin-Releasing Hormone