Involvement of gp130/interleukin-6 receptor transducing component in interleukin-11 receptor

Eur J Immunol. 1994 Jan;24(1):277-80. doi: 10.1002/eji.1830240143.


The recently cloned interleukin (IL)-11 displays many biological properties in common with those reported for IL-6. In order to analyze the nature and the functionality of the IL-11 receptor we developed a proliferative assay using the human multifactor-dependent cell line TF1. We showed that a blocking monoclonal antibody GPX7 raised against the gp130/IL-6 receptor transducing subunit was also able to inhibit the IL-11-triggered TF1 line proliferation. In addition, involvement of gp130 in IL-11 signaling was demonstrated by an induction of the transducing protein phosphorylation in response to IL-11, as observed for IL-6. In contrast, the blocking monoclonal antibody B-R6, which recognized the gp80/IL-6 binding subunit failed to interfere with the IL-11 proliferative signal in the TF1 cell line. Similarly, we did not observe any competition between IL-6 and IL-11 for a putative common binding site on the cell surface. These results suggest that the IL-11 binding component is different from the gp80/IL-6 receptor. In conclusion, IL-11, along with IL-6, leukemia inhibitory factor, oncostatin M and ciliary neurotrophic factor, belongs to the same family of cytokines, using gp130 as a transducing protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antigens, CD*
  • Cell Division / immunology
  • Cytokine Receptor gp130
  • Humans
  • Immunoblotting
  • Interleukin-11 / physiology*
  • Interleukin-11 Receptor alpha Subunit
  • Lymphocyte Activation
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / physiology*
  • Protein Binding
  • Receptors, Interleukin / physiology*
  • Receptors, Interleukin-11
  • Receptors, Interleukin-6
  • Signal Transduction / immunology*
  • Tumor Cells, Cultured


  • Antibodies, Monoclonal
  • Antigens, CD
  • IL11RA protein, human
  • IL6ST protein, human
  • Interleukin-11
  • Interleukin-11 Receptor alpha Subunit
  • Membrane Glycoproteins
  • Receptors, Interleukin
  • Receptors, Interleukin-11
  • Receptors, Interleukin-6
  • Cytokine Receptor gp130