Cholestasis caused by inhibition of the adenosine triphosphate-dependent bile salt transport in rat liver

Gastroenterology. 1994 Jul;107(1):255-65. doi: 10.1016/0016-5085(94)90084-1.


Background/aims: Inhibition of bile salt transport across the hepatocyte during cholestasis induced by cyclosporin A has been shown. However, the contribution of the different bile salt transport systems in liver to cholestasis has remained controversial.

Methods: The sensitivity of different bile salt transport systems in liver to cyclosporin-induced inhibition was determined by transport assays in plasma membrane vesicles and by in vivo studies in the rat.

Results: Cyclosporin A--induced inhibition of sodium-dependent uptake of bile salts across the sinusoidal membrane, of potential-dependent, and of adenosine triphosphate (ATP)-dependent bile salt transport across the canalicular membrane exhibited inhibition constants (Ki) of 5, 70, and 0.2 mumol/L, respectively. The nonimmunosuppressive cyclosporin analogue PSC 833 also preferentially inhibited the ATP-dependent bile salt transport with an inhibition constant of 0.6 mumol/L. Cyclosporin A and its analogue PSC 833 [(3'-oxo-4-butenyl-4-methyl-Thr1)-(Val2)-cyclosporin] (25 mg/kg each) served as tools to interfere with [14C]taurocholate secretion into bile in vivo, causing an accumulation of [14C]-taurocholate in liver and reducing bile flow to 50%. In mutant rats deficient in the transport of leukotriene C4 and related conjugates across the canalicular membrane, bile flow was reduced to 14%.

Conclusions: The cyclosporins preferentially inhibit the ATP-dependent bile salt export carrier in the canalicular membrane. This inhibition reduces bile salt-dependent bile flow and causes intrahepatic cholestasis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / physiology*
  • Animals
  • Bile Acids and Salts / metabolism*
  • Bile Canaliculi / cytology
  • Bile Canaliculi / metabolism
  • Bile Canaliculi / pathology
  • Biological Transport / physiology
  • Carbon Radioisotopes
  • Cell Membrane / metabolism
  • Cell Membrane / physiology
  • Cell Membrane / ultrastructure
  • Cholestasis / etiology*
  • Cholestasis / physiopathology
  • Cyclosporine / pharmacology
  • Cyclosporins / pharmacology
  • In Vitro Techniques
  • Leukotriene C4 / pharmacokinetics
  • Liver / cytology
  • Liver / metabolism*
  • Liver / physiology
  • Male
  • Rats
  • Rats, Wistar
  • Taurocholic Acid / pharmacology
  • Time Factors


  • Bile Acids and Salts
  • Carbon Radioisotopes
  • Cyclosporins
  • Leukotriene C4
  • Taurocholic Acid
  • Cyclosporine
  • Adenosine Triphosphate
  • valspodar