NG-nitro-L-arginine methyl ester inhibits the effect of an H3-histaminergic receptor agonist on NANC contraction in guinea-pig perfused bronchioles

J Pharm Pharmacol. 1994 Feb;46(2):153-5. doi: 10.1111/j.2042-7158.1994.tb03762.x.


A role for nitric oxide in the H3-histaminergic agonist-induced inhibition of the non-adrenergic, non-cholinergic (NANC) contraction has been studied in guinea-pig perfused bronchioles. (R)-alpha-Methylhistamine ((R)-alpha-MeHA), an agonist for H3 receptors, inhibited the NANC contraction induced by electrical field stimulation. NG-Nitro-L-arginine methyl ester (L-NAME) (50 microM), an inhibitor of nitric oxide synthesis, blocked the effect of (R)-alpha-MeHA. The effect of L-NAME was reversed by L-arginine (50 microM). L-NAME, L-arginine or (R)-alpha-MeHA were without effect on exogenous substance P- or neurokinin A-induced contractile responses of the perfused bronchioles. These results show that an H3-agonist inhibited the release of neurotransmitters in NANC nerve endings of guinea-pig perfused bronchioles presumably by production of nitric oxide.

MeSH terms

  • Animals
  • Arginine / analogs & derivatives*
  • Arginine / pharmacology
  • Bronchoconstriction / drug effects*
  • Electric Stimulation
  • Female
  • Guinea Pigs
  • Histamine Agonists / pharmacology*
  • Male
  • Methylhistamines / pharmacology*
  • NG-Nitroarginine Methyl Ester
  • Nitric Oxide / antagonists & inhibitors*
  • Receptors, Histamine H3 / drug effects*


  • Histamine Agonists
  • Methylhistamines
  • Receptors, Histamine H3
  • Nitric Oxide
  • alpha-methylhistamine
  • Arginine
  • NG-Nitroarginine Methyl Ester