Post-insult treatment with interleukin-1 receptor antagonist decreases oxidative lung injury in rats given intratracheal interleukin-1

Am J Respir Crit Care Med. 1994 Jul;150(1):109-12. doi: 10.1164/ajrccm.150.1.8025734.


Systemic administration of recombinant human interleukin-1 receptor antagonist (IL-1Ra) caused a rapid and sustained elevation of plasma IL-1Ra levels and decreased the leak of intravascularly injected 125I-labeled albumin into lungs of rats given human recombinant interleukin-1 intratracheally. IL-1Ra treatment decreased leak when given 0.5 h before, 1.25 h after, or 2.5 h after IL-1 administration. Similarly, IL-1Ra treatment decreased lavage leukocytes and neutrophils when given 0.5 h before, 1.25 h after, or 2.5 h after IL-1 administration. Pretreatment with IL-1Ra also decreased lung myeloperoxidase activity and breath H2O2 concentration increases in rats given IL-1 intratracheally. Our results suggest that IL-1Ra treatment may decrease acute lung injury and neutrophil influx even when given after an IL-1 inciting insult.

MeSH terms

  • Animals
  • Breath Tests
  • Bronchoalveolar Lavage Fluid / cytology
  • Hydrogen Peroxide / analysis
  • Interleukin-1 / physiology*
  • Lung / enzymology
  • Lung / pathology
  • Lung / physiopathology
  • Male
  • Neutrophils / pathology
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Interleukin-1 / antagonists & inhibitors*
  • Recombinant Proteins
  • Respiratory Distress Syndrome / pathology
  • Respiratory Distress Syndrome / physiopathology*
  • Respiratory Distress Syndrome / therapy


  • Interleukin-1
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Hydrogen Peroxide
  • Peroxidase