Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G alpha q and not G alpha 11

FEBS Lett. 1994 Jul 4;348(1):89-92. doi: 10.1016/0014-5793(94)00570-2.

Abstract

G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G alpha q and G alpha 11 and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. G alpha q antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). G alpha 11 antisense had little effect on either GRP-R- or NMB-R-mediated responses. This suggests that NMB-R couples to G alpha q, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Cloning, Molecular
  • GTP-Binding Proteins / metabolism*
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / pharmacology
  • Oocytes
  • Receptors, Bombesin / genetics
  • Receptors, Bombesin / metabolism*
  • Xenopus laevis

Substances

  • Oligonucleotides, Antisense
  • Receptors, Bombesin
  • GTP-Binding Proteins

Associated data

  • GENBANK/U10494
  • GENBANK/U10502